| Ada Wong | female | | Leon stumbles upon this mysterious woman who is quite secretive yet, at the same time, a professional and skilled investigator. |
| Albert Wesker | male | | Wesker has risen quickly inside the S.T.A.R.S. organization and currently leads the Alpha Team. |
| Alexia Ashford | female | | The first of the twins born through Alexander’s experiment in creating genius'. |
| Alfred Ashford | male | | Head of the Ashford legacy. |
| Alyssa Ashcroft | female | | A reporter for the Raccoon Tribune |
| Andy Holland | male | | Andy Holland is a underground supervisor for Umbrella's operations on Sheena Island. |
| Annette Birkin | female | | Annette is a researcher at Umbrella, working with her husband William on the next in line of Umbrella virus' - the G-Virus. |
| Ark Thompson | male | | Ark Thompson is the main character of Resident Evil Survivor. |
| Barry Burton | male | | Chris Redfield's old friend and partner. |
| Ben Bertolucci | male | | Ben is a reporter for one of the Raccoon City newspapers. |
| Billy Coen | male | | Convicted on circumstantual evidence, this ex-marine was sentanced to death for the murder of 23 people. |
| Brad Vickers | male | | Brad is a computer expert and excels in information gathering. |
| Brian Irons | male | | Brian Irons is the police chief of the Raccoon city Police Department. |
| Bruce McGivern | male | | Bruce is a member of an anti-Umbrella group. |
| Carlos Oliveira | male | | Carlos is from South America. His exact nationality is unknown but he carries Indian blood. |
| Chris Redfield | male | | After being kicked out of the Air Force, Chris became a drifter until he met Barry Burton. Barry recruited him for the newly formed S.T.A.R.S. |
| Cindy Lennox | female | | Cindy is a waitress at J's Bar. |
| Claire Redfield | female | * | The younger sister of Chris Redfield, Claire is a light-hearted, articulate modern woman. |
| Dario Rosso | male | | Dario is a salesman in Raccoon City and one of the survivors of the incident that Jill Valentine comes across. |
| David King | male | | David, a plumber is rumored to have a violent past. |
| Edward Dewey | male | | The mysterious Edward Dewey. |
| Enrico Marini | male | | Bravo Team's leader, and Wesker's second in command for the S.T.A.R.S. unit. |
| Enrico Marini | male | | The leader of Bravo Team and Rebecca's commanding officer. |
| Fong Ling | female | | Fong Ling is a covert Chinese government agent sent to hunt down forces working against the government. |
| Forest Speyer | male | | Explosives expert. |
| George Hamilton | male | | George is a doctor who works at the Raccoon City Hopsital. |
| Hunk | male | | One of Umbrella's top agents |
| James Marcus | male | | Head of the Umbrella Training Facility located on the other side of the Raccoon Forest. |
| Jill Valentine | female | | An intelligent soldier that has saved many S.T.A.R.S. members from danger in the past, Jill has been reassigned to Raccoon City just like Chris. |
| Jim Chapman | male | | Jim is a subway employee who has great powers of intuition and enjoys solving puzzles. |
| Joseph Frost | male | | Vehicle specialist and scout for STARS |
| Kenneth Sullivan | male | | A quiet but very talented field scouting officer. |
| Kevin Dooley | male | | Helicopter pilot for Bravo team. |
| Kevin Ryman | male | | Kevin is a member of the Raccoon City Police. |
| Leon S. Kennedy | male | | Leon is an idealistic rookie cop. |
| Lily Klein | female | | Lily is the daughter of some of the Umbrella workers on Sheena Island. |
| Lott Klein | male | | Lott is the son of two of the Umbrella researchers on Sheena Island. |
| Lucia | female | | A mysterious young girl found on the ship. |
| Mark Wilkins | male | | Mark is a security officer who also served in Vietnam. |
| Marvin Branagh | male | | Marvin is one of the officers located within the R.P.D. |
| Mikhail Victor | male | | Mikhail is a leader in the Umbrella Biohazard Countermeasure Squad. |
| Morpheus Duvall | male | | A researcher with Umbrella. |
| Murphy | male | | An UBCS solider. |
| Nemesis | | | Umbrella Europe's latest creation. |
| Nicholai Ginovaef | male | | Nicholai is one of the team sergeants in the Umbrella Biohazard Countermeasure Force. |
| Rebecca Chambers | female | | The youngest member of the group, Rebecca has been recruited for her knowledge of field medicine and first aid. |
| Richard Aiken | male | | A very important member of S.T.A.R.S. serving as Bravo's communications expert. |
| Rodrigo Juan Raval | male | | Part of the general Umbrella Security branch, Rodrigo works at the Umbrella HQ facility in France. |
| Sherry Birkin | female | | A lonely and insecure 12-year old, Sherry is quite mature for her age. |
| Steve Burnside | male | | Though young and often reckless, Steve is a good-hearted kid. |
| The Red Queen | female | | The Red Queen is the A.I. hologram of the Hive’s computer defense system. |
| Tyrell | male | | Like Nicholai he was sent in to gather data |
| Vincent Goldman | male | | Vincent Goldman is the commander of Sheena Island. |
| William Birkin | male | | William Birkin, father of Sherry and lead Umbrella Researcher at the new facility in Raccoon City |
| Yoko Suzuki | female | | Yoko is the computer expert who is attending Raccon University. |
